Article
In vitro genetic analysis of the Tetrahymena self-splicing intron.
Nature - 27 Sept 1990
Green R, Ellington A D, Szostak J W
Abstract excerpt
The availability of methods for the amplification of nucleic acid sequences allows the genetic analysis in vitro of the structural and functional properties of many nucleic acids. We have now developed an in vitro selection and amplification system, and used it to analyse the self-splicing Tetrah...
Topics
- Animals
- Bacterial Proteins
- Base Composition
- Base Sequence
- Cloning, Molecular
- DNA
- Deoxyribonuclease EcoRI
- Deoxyribonucleases, Type II Site-Specific
- Genetic Variation
- Introns
- Molecular Sequence Data
- Molecular Structure
- Polymerase Chain Reaction
- RNA, Catalytic
- Tetrahymena
