Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

In vitro genetic analysis of the Tetrahymena self-splicing intron.

Nature - 27 Sept 1990

Green R, Ellington A D, Szostak J W

Abstract excerpt

The availability of methods for the amplification of nucleic acid sequences allows the genetic analysis in vitro of the structural and functional properties of many nucleic acids. We have now developed an in vitro selection and amplification system, and used it to analyse the self-splicing Tetrahymena ribozyme. A much wider range of selective conditions can be used in vitro than is possible with standard in vivo...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacterial Proteins
Share to Base Sequence
Share to Cloning, Molecular

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genetic Variation

Share to Introns

Share to Molecular Sequence Data

Share to Polymerase Chain Reaction

Source

Identifiers

PMID: 2215650
DOI: 10.1038/347406a0

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

In vitro genetic analysis of the Tetrahymena self-splicing intron.PMID 2215650Read ↗

Selection in vitro of an RNA enzyme that specifically cleaves single-stranded DNA.Semantic proximity Read ↗

Evolution in vitro: analysis of a lineage of ribozymes.Semantic proximity Read ↗

Evolution of Tetrahymena ribozyme mutants with increased structural stability.Semantic proximity Read ↗

Specialization of the DNA-cleaving activity of a group I ribozyme through in vitro evolution.Semantic proximity Read ↗

The Tetrahymena ribozyme acts like an RNA restriction endonuclease.Semantic proximity Read ↗

Increased efficiency of evolved group I intron spliceozymes by decreased side product formation.Semantic proximity Read ↗

In vivo selection of better self-splicing introns in Escherichia coli: the role of the P1 extension helix of the Tetrahymena intron.Semantic proximity Read ↗

Alternative secondary structures in the 5' exon affect both forward and reverse self-splicing of the Tetrahymena intervening sequence RNA.Semantic proximity Read ↗

Evolutionary optimization of the catalytic properties of a DNA-cleaving ribozyme.Semantic proximity Read ↗

Role of a conserved J8/7 X P4 base-triple in the Tetrahymena ribozyme.Semantic proximity Read ↗

In vivo evolution of a catalytic RNA couples trans-splicing to translation.Semantic proximity Read ↗

Two universally conserved adenosines of the group I intron that are important for self-splicing but not for core catalytic activity.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.