Two universally conserved adenosines of the group I intron that are important for self-splicing but not for core catalytic activity.

Abstract excerpt

Two adenosine residues, universally conserved among group I introns, are located in the L4 region of the catalytic core. Base-substitution mutations in these adenosines resulted in diminished in vitro self-splicing activity of the Tetrahymena group I intron, more severely for double than for single mutations. The defect caused by the mutation of the L4 adenosines was manifest at the first step of splicing...

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