Article
Mutations in the Tetrahymena ribozyme internal guide sequence: effects on docking of the P1 helix into the catalytic core and correlation with catalytic activity.
Biochemistry - 3 Sept 1996
Campbell T B, Cech T R
Abstract excerpt
Binding of substrate by the ribozyme derived from the self-splicing intron of Tetrahymena thermophila involves at least two steps. In the first step, base pairing between the ribozyme internal guide sequence (IGS) and the substrate forms a helical duplex (P1). Through specific tertiary interactio...
Topics
- Animals
- Base Composition
- Base Sequence
- Binding Sites
- Catalysis
- Kinetics
- Mutation
- Nucleic Acid Conformation
- Oligoribonucleotides
- RNA Splicing
- RNA, Catalytic
- Tetrahymena thermophila