Article
In vivo selection of better self-splicing introns in Escherichia coli: the role of the P1 extension helix of the Tetrahymena intron.
RNA (New York, N.Y.) - 1 May 2002
Guo Feng, Cech Thomas R
Abstract excerpt
In vivo selection was used to improve the activity of the Tetrahymena pre-rRNA self-splicing intron in the context of heterologous exons. The intron was engineered into a kanamycin nucleotidyltransferase gene, with the pairing between intron bases and the 5' and 3' splice sites maintained. The initial construct failed to confer kanamycin resistance on Escherichia coli, although the pre-mRNA was active in splicing...
Topics
- Animals
- Base Sequence
- Escherichia coli
- Genetic Engineering
- Genetic Variation
- Guanosine Triphosphate
- Introns
- Kanamycin Resistance
- Kinetics
- Mutation
- Nucleotidyltransferases