Article
In vivo evolution of a catalytic RNA couples trans-splicing to translation.
PloS one - 1 Jan 2014
Olson Karen E, Dolan Gregory F, Müller Ulrich F
Abstract excerpt
How does a non-coding RNA evolve in cells? To address this question experimentally we evolved a trans-splicing variant of the group I intron ribozyme from Tetrahymena over 21 cycles of evolution in E.coli cells. Sequence variation was introduced during the evolution by mutagenic and recombinative PCR, and increasingly active ribozymes were selected by their repair of an mRNA mediating antibiotic resistance. The...
Topics
- Base Sequence
- Chloramphenicol
- Chloramphenicol O-Acetyltransferase
- Drug Resistance, Microbial
- Escherichia coli
- Evolution, Molecular
- Introns
- Molecular Sequence Data
- Mutation
- Protein Biosynthesis
- RNA Splicing
- RNA, Catalytic
- RNA, Messenger
- Real-Time Polymerase Chain Reaction
- Reverse Transcriptase Polymerase Chain Reaction
- Tetrahymena
- Trans-Splicing
- Transcription, Genetic