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Article

In vivo evolution of a catalytic RNA couples trans-splicing to translation.

PloS one - 1 Jan 2014

Olson Karen E, Dolan Gregory F, Müller Ulrich F

Abstract excerpt

How does a non-coding RNA evolve in cells? To address this question experimentally we evolved a trans-splicing variant of the group I intron ribozyme from Tetrahymena over 21 cycles of evolution in E.coli cells. Sequence variation was introduced during the evolution by mutagenic and recombinative PCR, and increasingly active ribozymes were selected by their repair of an mRNA mediating antibiotic resistance. The...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 24466112
DOI: 10.1371/journal.pone.0086473
PMCID: PMC3900562

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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In vivo evolution of a catalytic RNA couples trans-splicing to translation.PMID 24466112Read ↗

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