Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Comparison of non-canonical PAMs for CRISPR/Cas9-mediated DNA cleavage in human cells.

Scientific reports - 23 Jun 2014

Zhang Yilan, Ge Xianglian, Yang Fayu, Zhang Liping, Zheng Jiayong, Tan Xuefang, Jin Zi-Bing, Qu Jia, Gu Feng

Abstract excerpt

CRISPR/Cas9-mediated DNA cleavage (CCMDC) is becoming increasingly used for efficient genome engineering. Proto-spacer adjacent motif (PAM) adjacent to target sequence is one of the key components in the design of CCMDC strategies. It has been reported that NAG sequences are the predominant non-canonical PAM for CCMDC at the human EMX locus, but it is not clear whether it is universal at other loci. In the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Clustered Regularly Interspaced Short Palindromic Repeats
Share to CRISPR-Associated Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to CRISPR-Cas Systems

Share to DNA Cleavage

Share to Green Fluorescent Proteins

Share to HEK293 Cells

Share to Nucleotide Motifs

Share to Sequence Homology, Nucleic Acid

Source

Open in PubMed Open full text

Identifiers

PMID: 24956376
DOI: 10.1038/srep05405
PMCID: PMC4066725

Genes

PAM

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Comparison of non-canonical PAMs for CRISPR/Cas9-mediated DNA cleavage in human cells.PMID 24956376Read ↗

Closely related type II-C Cas9 orthologs recognize diverse PAMsSemantic proximity Read ↗

Diverse noncanonical PAMs recognized by SpCas9 in human cellsSemantic proximity Read ↗

Expanding PAM recognition and enhancing base editing activity of Cas9 variants with non-PI domain mutations derived from xCas9.Semantic proximity Read ↗

High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation.Semantic proximity Read ↗

GenomePAM directs PAM characterization and engineering of CRISPR-Cas nucleases using mammalian genome repeatsSemantic proximity Read ↗

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity.Semantic proximity Read ↗

Molecular engineering and dynamic activation mechanism of a PAM-flexible compact CRISPR-Cas9Semantic proximity Read ↗

Engineering of Cas12a nuclease variants with enhanced genome-editing specificitySemantic proximity Read ↗

SNPD-CRISPR: Single Nucleotide Polymorphism-Distinguishable Repression or Enhancement of a Target Gene Expression by CRISPR System.Semantic proximity Read ↗

Genome editing with natural and engineered CjCas9 orthologs.Semantic proximity Read ↗

Enhancement of Target Specificity of CRISPR-Cas12a by Using a Chimeric DNA-RNA GuideSemantic proximity Read ↗

Engineering a PAM-flexible SpdCas9 variant as a universal gene repressor.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.