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Article

Engineering of Cas12a nuclease variants with enhanced genome-editing specificity

14 Mar 2024

Abstract excerpt

The clustered regularly interspaced short palindromic repeat (CRISPR)-Cas12a system is a powerful tool in gene editing; however, crRNA-DNA mismatches might induce unwanted cleavage events, especially at the distal end of the PAM. To minimize this limitation, we engineered a hyper fidelity AsCas12a variant carrying the mutations S186A/R301A/T315A/Q1014A/K414A (termed HyperFi-As) by modifying amino acid residues...

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PMID: 38483978
DOI: 10.1371/journal.pbio.3002514

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