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Article

Genome editing with natural and engineered CjCas9 orthologs.

Molecular therapy : the journal of the American Society of Gene Therapy - 5 Apr 2023

Gao Siqi, Wang Yao, Qi Tao, Wei Jingjing, Hu Ziying, Liu Jingtong, Sun Shuna, Liu Huihui, Wang Yongming

Abstract excerpt

CjCas9 is one of the smallest CRISPR-associated (Cas9) nucleases for mammalian genome editing. However, it requires a long N4RYAC (R = A or G; Y = C or T) protospacer-adjacent motif (PAM), limiting its DNA targeting scope. In this study, we investigated the PAMs of three CjCas9 orthologs, including Hsp1Cas9, Hsp2Cas9, and CcuCas9, by performing a GFP-activation assay. Interestingly, Hsp1Cas9 and CcuCas9...

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Identifiers

PMID: 36733251
DOI: 10.1016/j.ymthe.2023.01.029
PMCID: PMC10124074

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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