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Article

High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation.

Cell reports - 3 Mar 2020

Legut Mateusz, Daniloski Zharko, Xue Xinhe, McKenzie Dayna, Guo Xinyi, Wessels Hans-Hermann, Sanjana Neville E

Abstract excerpt

A key limitation of the widely used CRISPR enzyme S. pyogenes Cas9 is the strict requirement of an NGG protospacer-adjacent motif (PAM) at the target site. This constraint can be limiting for genome editing applications that require precise Cas9 positioning. Recently, two Cas9 variants with a relaxed PAM requirement (NG) have been developed (xCas9 and Cas9-NG), but their activity has been measured at only a small...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 32130891
DOI: 10.1016/j.celrep.2020.02.010
PMCID: PMC7558435

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