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Enhancement of Target Specificity of CRISPR-Cas12a by Using a Chimeric DNA-RNA Guide

2020-02-04

Abstract excerpt

The CRISPR-Cas9 system is widely used for target-specific genome engineering. Cpf1 is one of the CRISPR effectors that controls target genes by recognizing thymine-rich protospacer adjacent motif (PAM) sequences. Cpf1 has a higher sensitivity to mismatches in the guide RNA than does Cas9; therefore, off-target sequence recognition and cleavage are lower. However, it tolerates mismatches in regions distant from the...

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Literature Corpus work
be5dfb1f-e157-59b9-a7c7-81564543cdbc
DOI
10.1101/2020.02.04.933614
Open publication

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Enhancement of Target Specificity of CRISPR-Cas12a by Using a Chimeric DNA-RNA GuideDOI 10.1101/2020.02.04.933614
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