Article
Evolved Cas9 variants with broad PAM compatibility and high DNA specificity.
Nature - 5 Apr 2018
Hu Johnny H, Miller Shannon M, Geurts Maarten H, Tang Weixin, Chen Liwei, Sun Ning, Zeina Christina M, Gao Xue, Rees Holly A, Lin Zhi, Liu David R
Abstract excerpt
A key limitation of the use of the CRISPR-Cas9 system for genome editing and other applications is the requirement that a protospacer adjacent motif (PAM) be present at the target site. For the most commonly used Cas9 from Streptococcus pyogenes (SpCas9), the required PAM sequence is NGG. No natural or engineered Cas9 variants that have been shown to function efficiently in mammalian cells offer a PAM less...
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