Article
Expanding PAM recognition and enhancing base editing activity of Cas9 variants with non-PI domain mutations derived from xCas9.
The FEBS journal - 1 Oct 2022
Xie Lifang, Hu Yun, Li Li, Jiang Lurong, Jiao Yaoge, Wang Yanhong, Zhou Lifang, Tao Rui, Qu Junyan, Chen Qiang, Yao Shaohua
Abstract excerpt
The recognition of protospacer adjacent motif (PAM) is a key factor for the CRISPR (i.e. clustered regularly interspaced short palindromic repeats)/CRISPR-associated 9 (Cas9) system to distinguish foreign DNAs from the host genome, and also significantly restricts the targeting scope of the system during genome-editing applications. Structurally, the PAM interacting (PI) domain, which usually is located in the...
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