Article
A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities.
Molecular systems biology - 8 Oct 2013
Vizeacoumar Franco J, Arnold Roland, Vizeacoumar Frederick S, Chandrashekhar Megha, Buzina Alla, Young Jordan T F, Kwan Julian H M, Sayad Azin, Mero Patricia, Lawo Steffen, Tanaka Hiromasa, Brown Kevin R, Baryshnikova Anastasia, Mak Anthony B, Fedyshyn Yaroslav, Wang Yadong, Brito Glauber C, Kasimer Dahlia, Makhnevych Taras, Ketela Troy, Datti Alessandro, Babu Mohan, Emili Andrew, Pelletier Laurence, Wrana Jeff, Wainberg Zev, Kim Philip M, Rottapel Robert, O'Brien Catherine A, Andrews Brenda, Boone Charles, Moffat Jason
Abstract excerpt
Improved efforts are necessary to define the functional product of cancer mutations currently being revealed through large-scale sequencing efforts. Using genome-scale pooled shRNA screening technology, we mapped negative genetic interactions across a set of isogenic cancer cell lines and confirmed hundreds of these interactions in orthogonal co-culture competition assays to generate a high-confidence genetic...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.