Article
DNA sequence analysis of mutations induced by N-2-acetylamino-7-iodofluorene in plasmid pBR322 in Escherichia coli.
Journal of molecular biology - 20 May 1990
Hoffmann G R, Fuchs R P
Abstract excerpt
The spectrum of mutations induced by N-2-acetylamino-7-iodofluorene (AAIF) was analyzed in a forward mutation system based on mutagenesis directed to a small restriction fragment in the tetracycline resistance gene of plasmid pBR322. AAIF was found to induce frameshift mutations and base-pair substitutions at approximately equal frequencies. The frameshift mutations were mostly deletions of single base-pairs, but...
Topics
- 2-Acetylaminofluorene
- Base Sequence
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Mutation
- Plasmids
- Tetracycline Resistance
- Transformation, Bacterial