Construction of frameshift mutation hot spots within the tetracycline resistance gene of pBR322.

doi:10.1016/s0300-9084(85)80085-7

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Article

Construction of frameshift mutation hot spots within the tetracycline resistance gene of pBR322.

Biochimie - 1 Jan 2000

Burnouf D, Fuchs R P

Abstract excerpt

The chemical carcinogen, N-2-acetylaminofluorene (AAF) when bound covalently to DNA induces a majority (greater than 90%) of frameshift mutations. The mutations occur with high frequencies at defined sequences (i.e. mutation hot spots). Two classes of mutation hot spots were found: at repetitive...

Topics

2-Acetylaminofluorene
Base Sequence
DNA Restriction Enzymes
DNA, Bacterial
Deoxyribonucleases, Type II Site-Specific
Genes, Bacterial
Mutation
R Factors
Repetitive Sequences, Nucleic Acid
Tetracycline
Ultraviolet Rays

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