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Improved gene correction efficiency with a tailed duplex DNA fragment.

Biochemistry - 19 Aug 2008

Tsuchiya Hiroyuki, Uchiyama Masayuki, Hara Kazuhiro, Nakatsu Yoshimichi, Tsuzuki Teruhisa, Inoue Hideo, Harashima Hideyoshi, Kamiya Hiroyuki

Abstract excerpt

A 606-base single-stranded (ss) DNA fragment, prepared by restriction enzyme digestion of ss phagemid DNA, corrects a hygromycin resistance and enhanced green fluorescent protein (Hyg-EGFP) fusion gene more efficiently than a PCR fragment, which is the conventional type of DNA fragment used in gene correction. Here, a tailed duplex, obtained by annealing an oligonucleotide to the ss DNA fragment, was used in the...

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PMID: 18642931
DOI: 10.1021/bi800588k

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RAD51

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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