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Article

Identification of factors influencing strand bias in oligonucleotide-mediated recombination in Escherichia coli.

Nucleic acids research - 15 Nov 2003

Li Xin-tian, Costantino Nina, Lu Lin-yu, Liu De-pei, Watt Rory M, Cheah Kathryn S E, Court Donald L, Huang Jian-Dong

Abstract excerpt

Recombinogenic engineering methodology, also known as recombineering, utilizes homologous recombination to create targeted changes in cellular DNA with great specificity and flexibility. In Escherichia coli, the Red recombination system from bacteriophage lambda has been used successfully to modify both plasmid and chromosomal DNA in a highly efficient manner, using either a linear double-stranded DNA fragment or...

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Identifiers

PMID: 14602928
DOI: 10.1093/nar/gkg844
PMCID: PMC275540

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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