Article
An improved recombineering approach by adding RecA to lambda Red recombination.
Molecular biotechnology - 1 Jan 2006
Wang Junping, Sarov Mihail, Rientjes Jeanette, Fu Jun, Hollak Heike, Kranz Harald, Xie Wei, Stewart A Francis, Zhang Youming
Abstract excerpt
Recombineering is the use of homologous recombination in Escherichia coli for DNA engineering. Of several approaches, use of the lambda phage Red operon is emerging as the most reliable and flexible. The Red operon includes three components: Redalpha, a 5' to 3' exonuclease, Redbeta, an annealing protein, and Redgamma, an inhibitor of the major E. coli exonuclease and recombination complex, RecBCD. Most E. coli...
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