Article
Factors affecting SFHR gene correction efficiency with single-stranded DNA fragment.
Biochemical and biophysical research communications - 4 Nov 2005
Tsuchiya Hiroyuki, Harashima Hideyoshi, Kamiya Hiroyuki
Abstract excerpt
A 606-nt single-stranded (ss) DNA fragment, prepared by restriction enzyme digestion of ss phagemid DNA, improves the gene correction efficiency by 12-fold as compared with a PCR fragment, which is the conventional type of fragment used in the small fragment homologous replacement method [H. Tsuchiya, H. Harashima, H. Kamiya, Increased SFHR gene correction efficiency with sense single-stranded DNA, J. Gene Med. 7...
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