Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Improvement of recombination efficiency by mutation of red proteins.

BioTechniques - 1 Jun 2005

Nakayama Manabu, Ohara Osamu

Abstract excerpt

The Red recombination system of the bacteriophage lambda is a useful tool for engineering bacterial artificial chromosomes (BACs) because desired mutations can be made in any part of the DNA, even in large DNAs, independent of the presence of appropriate restriction enzyme sites. Many have tried changing the inducible promoter or decreasing the copy number of Red genes in Escherichia coli to improve the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to 5' Untranslated Regions
Share to Bacteriophage lambda
Share to Chromosomes, Artificial, Bacterial

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA Primers

Share to Electrophoresis, Polyacrylamide Gel

Share to Plasmids

Share to Recombination, Genetic

Share to Viral Proteins

Source

Identifiers

PMID: 16018553
DOI: 10.2144/05386RR02

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Improvement of recombination efficiency by mutation of red proteins.PMID 16018553Read ↗

An improved recombineering approach by adding RecA to lambda Red recombination.Semantic proximity Read ↗

Modifying bacteriophage lambda with recombineering.Semantic proximity Read ↗

Lambda red recombineering in Escherichia coli occurs through a fully single-stranded intermediate.Semantic proximity Read ↗

Enhanced levels of lambda Red-mediated recombinants in mismatch repair mutants.Semantic proximity Read ↗

Involvement of Escherichia coli DNA Replication Proteins in Phage Lambda Red-Mediated Homologous Recombination.Semantic proximity Read ↗

Improved genetic selection for screening bacteriophage libraries by homologous recombination in vivo.Semantic proximity Read ↗

Improving lambda red genome engineering in Escherichia coli via rational removal of endogenous nucleases.Semantic proximity Read ↗

Lambda Red-mediated recombinogenic engineering of enterohemorrhagic and enteropathogenic E. coli.Semantic proximity Read ↗

Optimization of a Lambda-RED Recombination Method for Rapid Gene Deletion in Human Cytomegalovirus.Semantic proximity Read ↗

Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli.Semantic proximity Read ↗

Donor plasmids for phenotypically neutral chromosomal gene insertions in Enterobacteriaceae.Semantic proximity Read ↗

Application of lambda Red recombination system to Vibrio cholerae genetics: simple methods for inactivation and modification of chromosomal genes.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.