Effects of target sequence and sense versus anti-sense strands on gene correction with single-stranded DNA fragments.

Abstract excerpt

The correction of an inactivated hygromycin resistance and enhanced green fluorescent protein (Hyg-EGFP) fusion gene by a several hundred-base single-stranded (ss) DNA fragment has been reported. In this study, the effectiveness of this type of gene correction was examined for various positions in the rpsL gene. Sense and anti-sense ssDNA fragments were prepared, and the gene correction efficiencies were...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Artificial Gene Fusion
• Share to Cinnamates