Article
Targeted correction of a chromosomal point mutation by modified single-stranded oligonucleotides in a GFP recovery system.
Biochemical and biophysical research communications - 9 Sept 2005
Yin Wen-Xuan, Wu Xue-Song, Liu Guang, Li Zhu-Hong, Watt Rory M, Huang Jian-Dong, Liu De-Pei, Liang Chih-Chuan
Abstract excerpt
Synthetic oligonucleotides had been employed in DNA repair and promised great potentials in gene therapy. To test the ability of single-stranded oligonucleotide (SSO)-mediated gene repair within a chromosomal site in human cells, a HeLa cell line stably integrated with mutant enhanced green fluorescence protein gene (mEGFP) in the genome was established. Transfection with specific SSOs successfully repaired the...
Topics
- Chromosome Aberrations
- Electroporation
- Gene Targeting
- Gene Transfer Techniques
- Genetic Therapy
- Green Fluorescent Proteins
- HeLa Cells
- Humans
- Oligonucleotides
- Point Mutation
- Polymorphism, Single Nucleotide
- Transfection