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Article

Development of transgenic Daphnia magna for visualizing homology-directed repair of DNA.

Scientific reports - 15 Feb 2022

Fatimah Rizky Mutiara, Adhitama Nikko, Kato Yasuhiko, Watanabe Hajime

Abstract excerpt

In the crustacean Daphnia magna, studying homology-directed repair (HDR) is important to understand genome maintenance during parthenogenesis, effects of environmental toxicants on the genome, and improvement of HDR-mediated genome editing. Here we developed a transgenic D. magna that expresses green fluorescence protein (GFP) upon HDR occurrence. We utilized the previously established reporter plasmid named...

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Identifiers

PMID: 35169221
DOI: 10.1038/s41598-022-06526-8
PMCID: PMC8847417

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