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Article

Enhanced oligonucleotide-directed gene targeting in mammalian cells following treatment with DNA damaging agents.

Experimental cell research - 15 Oct 2004

Ferrara Luciana, Parekh-Olmedo Hetal, Kmiec Eric B

Abstract excerpt

Targeted gene repair, a form of oligonucleotide-directed mutagenesis, employs end-modified single-stranded DNA oligonucleotides to mediate single-base changes in chromosomal DNA. In this work, we use a specific 72-mer to direct the repair of a mutated eGFP gene stably integrated in the genome of DLD-1 cells. Corrected cells express eGFP that can be identified and quantitated by FACS. The repair of this mutant...

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Identifiers

PMID: 15383324
DOI: 10.1016/j.yexcr.2004.06.021

Genes

DLD

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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