Article
Methods for reverse genetic screening in zebrafish by resequencing and TILLING.
Methods (San Diego, Calif.) - 1 Jul 2006
Sood Raman, English Milton A, Jones MaryPat, Mullikin James, Wang Duen-Mei, Anderson Maria, Wu Dongying, Chandrasekharappa Settara C, Yu Jun, Zhang Jinghui, Paul Liu P
Abstract excerpt
Animal models provide an in vivo system to study gene function by transgenic and knockout approaches. Targeted knockout approaches have been very successful in mice, but are currently not feasible in zebrafish due to the inability to grow embryonic stem cells. As an alternative, a reverse genetic approach that utilizes screening by resequencing and/or TILLING (Targeting Induced Local Lesions INGenomes) of...
Topics
- Animals
- Computational Biology
- Cryopreservation
- DNA Mutational Analysis
- Ethylnitrosourea
- Female
- Fertilization in Vitro
- Gene Targeting
- Genome
- Male
- Mutagenesis
- Polymorphism, Single Nucleotide
- Semen Preservation