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Article

TILLING is an effective reverse genetics technique for Caenorhabditis elegans.

BMC genomics - 18 Oct 2006

Gilchrist Erin J, O'Neil Nigel J, Rose Ann M, Zetka Monique C, Haughn George W

Abstract excerpt

BACKGROUND: TILLING (Targeting Induced Local Lesions in Genomes) is a reverse genetic technique based on the use of a mismatch-specific enzyme that identifies mutations in a target gene through heteroduplex analysis. We tested this technique in Caenorhabditis elegans, a model organism in which genomics tools have been well developed, but limitations in reverse genetics have restricted the number of heritable...

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Identifiers

PMID: 17049087
DOI: 10.1186/1471-2164-7-262
PMCID: PMC1626091

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