Article
A large-scale zebrafish gene knockout resource for the genome-wide study of gene function.
Genome research - 1 Apr 2013
Varshney Gaurav K, Lu Jing, Gildea Derek E, Huang Haigen, Pei Wuhong, Yang Zhongan, Huang Sunny C, Schoenfeld David, Pho Nam H, Casero David, Hirase Takashi, Mosbrook-Davis Deborah, Zhang Suiyuan, Jao Li-En, Zhang Bo, Woods Ian G, Zimmerman Steven, Schier Alexander F, Wolfsberg Tyra G, Pellegrini Matteo, Burgess Shawn M, Lin Shuo
Abstract excerpt
With the completion of the zebrafish genome sequencing project, it becomes possible to analyze the function of zebrafish genes in a systematic way. The first step in such an analysis is to inactivate each protein-coding gene by targeted or random mutation. Here we describe a streamlined pipeline using proviral insertions coupled with high-throughput sequencing and mapping technologies to widely mutagenize genes...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.