Article
Rapid identification and recovery of ENU-induced mutations with next-generation sequencing and Paired-End Low-Error analysis.
BMC genomics - 14 Feb 2015
Pan Luyuan, Shah Arish N, Phelps Ian G, Doherty Dan, Johnson Eric A, Moens Cecilia B
Abstract excerpt
BACKGROUND: Targeting Induced Local Lesions IN Genomes (TILLING) is a reverse genetics approach to directly identify point mutations in specific genes of interest in genomic DNA from a large chemically mutagenized population. Classical TILLING processes, based on enzymatic detection of mutations in heteroduplex PCR amplicons, are slow and labor intensive. RESULTS: Here we describe a new TILLING strategy in...
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