Isolation of gonadal mutations in adult zebrafish from a chemical mutagenesis screen.

doi:10.1095/biolreprod64.2.548

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Article

Isolation of gonadal mutations in adult zebrafish from a chemical mutagenesis screen.

Biology of reproduction - 1 Feb 2001

Bauer M P, Goetz F W

Abstract excerpt

A mutagenesis screen was conducted on zebrafish using N:-ethyl N:-nitrosourea as a mutagen and an F2 crossing scheme to obtain homozygous mutants in the F3 generation. Whole abdomens of 3-mo-old F3 zebrafish progeny were fixed and mass-embedded in paraffin blocks. Blocks were cut with a microtome to obtain cross-sections of the entire body cavity that included the ovaries and testes. Slides of the cross-sections...

Topics

Animals
Drug Evaluation, Preclinical
Ethylnitrosourea
Female
Gonads
Male
Mutagenesis
Mutagens
Mutation
Oocytes
Ovarian Follicle
Ovary
Phenotype
Spermatogenesis
Testis
Zebrafish

Source

Open in PubMed

Identifiers

PMID: 11159357
DOI: 10.1095/biolreprod64.2.548

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