Article
Isolation of gonadal mutations in adult zebrafish from a chemical mutagenesis screen.
Biology of reproduction - 1 Feb 2001
Bauer M P, Goetz F W
Abstract excerpt
A mutagenesis screen was conducted on zebrafish using N:-ethyl N:-nitrosourea as a mutagen and an F2 crossing scheme to obtain homozygous mutants in the F3 generation. Whole abdomens of 3-mo-old F3 zebrafish progeny were fixed and mass-embedded in paraffin blocks. Blocks were cut with a microtome to obtain cross-sections of the entire body cavity that included the ovaries and testes. Slides of the cross-sections...
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