Article
New approach for fish breeding by chemical mutagenesis: establishment of TILLING method in fugu (Takifugu rubripes) with ENU mutagenesis.
BMC genomics - 13 Nov 2013
Kuroyanagi Miwa, Katayama Takashi, Imai Tadashi, Yamamoto Yoshihisa, Chisada Shin-ichi, Yoshiura Yasutoshi, Ushijima Tomokazu, Matsushita Tomonao, Fujita Masashi, Nozawa Aoi, Suzuki Yuzuru, Kikuchi Kiyoshi, Okamoto Hiroyuki
Abstract excerpt
BACKGROUND: In fish breeding, it is essential to discover and generate fish exhibiting an effective phenotype for the aquaculture industry, but screening for natural mutants by only depending on natural spontaneous mutations is limited. Presently, reverse genetics has become an important tool to generate mutants, which exhibit the phenotype caused by inactivation of a gene. TILLING (Targeting Induced Local...
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