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Article

Energetics of folding subtilisin BPN'.

Biochemistry - 2 Jun 1992

Bryan P, Alexander P, Strausberg S, Schwarz F, Lan W, Gilliland G, Gallagher D T

Abstract excerpt

Subtilisin is an unusual example of a monomeric protein with a substantial kinetic barrier to folding and unfolding. Here we document for the first time the in vitro folding of the mature form of subtilisin. Subtilisin was modified by site-directed mutagenesis to be proteolytically inactive, allowing the impediments to folding to be systematically examined. First, the thermodynamics and kinetics of calcium...

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Source

Identifiers

PMID: 1599918
DOI: 10.1021/bi00136a003

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Prodomain mutations at the subtilisin interface: correlation of binding energy and the rate of catalyzed folding.Semantic proximity Read ↗

Crystal structure analysis of subtilisin BPN' mutants engineered for studying thermal stability.Semantic proximity Read ↗

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