Article
Energetics of folding subtilisin BPN'.
Biochemistry - 2 Jun 1992
Bryan P, Alexander P, Strausberg S, Schwarz F, Lan W, Gilliland G, Gallagher D T
Abstract excerpt
Subtilisin is an unusual example of a monomeric protein with a substantial kinetic barrier to folding and unfolding. Here we document for the first time the in vitro folding of the mature form of subtilisin. Subtilisin was modified by site-directed mutagenesis to be proteolytically inactive, allowing the impediments to folding to be systematically examined. First, the thermodynamics and kinetics of calcium...
Topics
- Bacillus
- Calcium
- Calorimetry, Differential Scanning
- Cloning, Molecular
- Genes, Bacterial
- Kinetics
- Mutation
- Protein Conformation
- Spectrometry, Fluorescence
- Subtilisins
- Temperature
- Thermodynamics