Contribution of long-range electrostatic interactions to the stabilization of the catalytic transition state of the serine protease subtilisin BPN'.

doi:10.1021/bi00213a021

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Article

Contribution of long-range electrostatic interactions to the stabilization of the catalytic transition state of the serine protease subtilisin BPN'.

Biochemistry - 21 Dec 1993

Jackson S E, Fersht A R

Abstract excerpt

The possible role of long-range electrostatic interactions on the catalytic activity of the serine protease subtilisin BPN' is investigated using protein engineering techniques. Charged residues on the surface of the enzyme some 13-15 A from the active site were mutated to either neutral or oppos...

Topics

Bacillus
Catalysis
Electrochemistry
Enzyme Stability
Hydrolysis
Kinetics
Mutation
Oligopeptides
Substrate Specificity
Subtilisins

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