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Article

Structural basis of thermostability. Analysis of stabilizing mutations in subtilisin BPN'.

The Journal of biological chemistry - 26 Jul 2002

Almog Orna, Gallagher D T, Ladner Jane E, Strausberg Susan, Alexander Patrick, Bryan Philip, Gilliland Gary L

Abstract excerpt

The crystal structures of two thermally stabilized subtilisin BPN' variants, S63 and S88, are reported here at 1.8 and 1.9 A resolution, respectively. The micromolar affinity calcium binding site (site A) has been deleted (Delta75-83) in these variants, enabling the activity and thermostability measurements in chelating conditions. Each of the variants includes mutations known previously to increase the...

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Identifiers

PMID: 12011071
DOI: 10.1074/jbc.M111777200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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