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Article

Prediction of electrostatic effects of engineering of protein charges.

Nature - 1 Jan 2000

Sternberg M J, Hayes F R, Russell A J, Thomas P G, Fersht A R

Abstract excerpt

Accurate prediction of electrostatic effects on catalytic activity is an essential component of protein design. Site-directed mutagenesis of charged groups in subtilisin of Bacillus amyloliquefaciens has provided experimental measurements of electrostatic interactions which may be used to test such theoretical methods. The pKa of the histidine of the active site has been perturbed by +0.08 to -1.0 units by...

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PMID: 3313059
DOI: 10.1038/330086a0

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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