Prediction of electrostatic effects of engineering of protein charges.

doi:10.1038/330086a0

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Article

Prediction of electrostatic effects of engineering of protein charges.

Nature - 1 Jan 2000

Sternberg M J, Hayes F R, Russell A J, Thomas P G, Fersht A R

Abstract excerpt

Accurate prediction of electrostatic effects on catalytic activity is an essential component of protein design. Site-directed mutagenesis of charged groups in subtilisin of Bacillus amyloliquefaciens has provided experimental measurements of electrostatic interactions which may be used to test su...

Topics

Algorithms
Bacillus
Electrochemistry
Kinetics
Models, Theoretical
Mutation
Protein Conformation
Subtilisins

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