Cation-dependent stability of subtilisin.

doi:10.1021/bi010797m

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Article

Cation-dependent stability of subtilisin.

Biochemistry - 4 Sept 2001

Alexander P A, Ruan B, Bryan P N

Abstract excerpt

Subtilisin BPN' contains two cation binding sites. One specifically binds calcium (site A), and the other can bind both divalent and monovalvent metals (site B). By binding at specific sites in the tertiary structure of subtilisin, cations contribute their binding energy to the stability of the native state and increase the activation energy of unfolding. Deconvoluting the influence of binding sites A and B on...

Topics

Bacillus
Calcium
Cations
Cloning, Molecular
Enzyme Stability
Kinetics
Models, Molecular
Mutation
Protein Conformation
Sodium
Subtilisin

Source

Open in PubMed

Identifiers

PMID: 11524007
DOI: 10.1021/bi010797m

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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