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Article

Temperature-gradient gel electrophoresis for analysis and screening of thermostable proteases.

Electrophoresis - 1 Aug 1993

Sättler A, Riesner D

Abstract excerpt

The thermal unfolding of microbial serine proteases was studied by temperature-gradient gel electrophoresis (TGGE). Conditions for a native polyacrylamide gel electrophoresis were established, and the temperature gradient was applied perpendicularly to the direction of electrophoretic migration. Mobility changes of the protease molecules were indicative for thermally induced conformational changes. The transition...

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Identifiers

PMID: 8404822
DOI: 10.1002/elps.11501401122

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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