Article
Temperature-gradient gel electrophoresis for analysis and screening of thermostable proteases.
Electrophoresis - 1 Aug 1993
Sättler A, Riesner D
Abstract excerpt
The thermal unfolding of microbial serine proteases was studied by temperature-gradient gel electrophoresis (TGGE). Conditions for a native polyacrylamide gel electrophoresis were established, and the temperature gradient was applied perpendicularly to the direction of electrophoretic migration....
Topics
- Buffers
- Calcium
- Electrophoresis, Polyacrylamide Gel
- Endopeptidases
- Enzyme Stability
- Hot Temperature
- Isoflurophate
- Mutation
- Phenylmethylsulfonyl Fluoride
- Protein Conformation
- Serine Endopeptidases
- Serine Proteinase Inhibitors
- Subtilisins
- Temperature