Article
Protein engineering of subtilisin.
Biochimica et biophysica acta - 29 Dec 2000
Bryan P N
Abstract excerpt
The serine protease subtilisin is an important industrial enzyme as well as a model for understanding the enormous rate enhancements affected by enzymes. For these reasons along with the timely cloning of the gene, ease of expression and purification and availability of atomic resolution structures, subtilisin became a model system for protein engineering studies in the 1980s. Fifteen years later, mutations in...
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