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Article

Crystal structure of calcium-independent subtilisin BPN' with restored thermal stability folded without the prodomain.

Proteins - 1 Apr 1998

Almog O, Gallagher T, Tordova M, Hoskins J, Bryan P, Gilliland G L

Abstract excerpt

The three-dimensional structure of a subtilisin BPN' construct that was produced and folded without its prodomain shows the tertiary structure is nearly identical to the wild-type enzyme and not a folding intermediate. The subtilisin BPN' variant, Sbt70, was cloned and expressed in Escherichia coli without the prodomain, the 77-residue N-terminal domain that catalyzes the folding of the enzyme into its native...

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PMID: 9552156
DOI: 10.1002/(sici)1097-0134(19980401)31:1<21::aid-prot3>3.0.co;2-k

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