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Article

Engineering substrate preference in subtilisin: structural and kinetic analysis of a specificity mutant.

Biochemistry - 24 Jun 2008

Ruan Biao, London Viktoriya, Fisher Kathryn E, Gallagher D Travis, Bryan Philip N

Abstract excerpt

Bacillus subtilisin has been a popular model protein for engineering altered substrate specificity. Although some studies have succeeded in increasing the specificity of subtilisin, they also demonstrate that high specificity is difficult to achieve solely by engineering selective substrate binding. In this paper, we analyze the structure and transient state kinetic behavior of Sbt160, a subtilisin engineered to...

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Identifiers

PMID: 18507395
DOI: 10.1021/bi800089f

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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