Article
Prodomain mutations at the subtilisin interface: correlation of binding energy and the rate of catalyzed folding.
Biochemistry - 28 Nov 1995
Wang L, Ruvinov S, Strausberg S, Gallagher D T, Gilliland G, Bryan P N
Abstract excerpt
The in vivo folding of subtilisin is dependent on a 77 amino acid prosequence, which is eventually cleaved from the N-terminus of subtilisin to create the 275 amino acid mature form of the enzyme. The recent determination of the structure of a complex of the prodomain and a calcium-free subtilisi...
Topics
- Mutation
- Protein Binding
- Protein Folding
- Subtilisins