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Article

Crystal structure analysis of subtilisin BPN' mutants engineered for studying thermal stability.

Advances in experimental medicine and biology - 1 Jan 1996

Gilliland G L, Gallagher D T, Alexander P, Bryan P

Abstract excerpt

The high resolution crystal structures of four genetically engineered subtilisin BPN' variants (E.C. 3.4.21.14) which vary dramatically in their stability have been determined. The simplest variant, S3, contains two altered residues, N218S and S221C. The N218S change was incorporated for its stabilizing effects and its influence on crystallization; the S221C change, a modification of the active site serine, was...

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Identifiers

PMID: 8796321
DOI: 10.1007/978-1-4613-0319-0_18

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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