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Article

Crystal structure analysis of subtilisin BPN' mutants engineered for studying thermal stability.

Advances in experimental medicine and biology - 1 Jan 1996

Gilliland G L, Gallagher D T, Alexander P, Bryan P

Abstract excerpt

The high resolution crystal structures of four genetically engineered subtilisin BPN' variants (E.C. 3.4.21.14) which vary dramatically in their stability have been determined. The simplest variant, S3, contains two altered residues, N218S and S221C. The N218S change was incorporated for its stab...

Topics

Crystallography, X-Ray
Enzyme Stability
Mutation
Protein Conformation
Protein Engineering
Subtilisins
Temperature

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 8796321
DOI: 10.1007/978-1-4613-0319-0_18

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8796321-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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