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Article

Stabilizing mutations and calcium-dependent stability of subtilisin.

Biochemistry - 4 Sept 2001

Alexander P A, Ruan B, Strausberg S L, Bryan P N

Abstract excerpt

Stability is a property of subtilisin which has proven particularly amenable to enhancement via random mutagenesis and screening, yet the effects of most stabilizing mutations are not understood in structural and energetic detail. This paper seeks to explain the longstanding observation that stabilizing mutations are usually calcium-dependent in their stabilizing effect, irrespective of their proximity to the...

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PMID: 11524008
DOI: 10.1021/bi010798e

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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