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Article

DnaA dependent replication of plasmid R1 occurs in the presence of point mutations that disrupt the dnaA box of oriR.

Nucleic acids research - 25 May 1992

Ortega-Jiménez S, Giraldo-Suárez R, Fernández-Tresguerres M E, Berzal-Herranz A, Díaz-Orejas R

Abstract excerpt

We have found that DnaA dependent replication of R1 still occurred when 5 of the 9 bases in the dnaA box present in oriR were changed by site directed mutagenesis although the replication efficiency decreased to 20% and 70% of the wild-type origin in vitro and in vivo respectively. Additional mutation of a second dnaA box, 28 bp upstream oriR, that differs in only one base from the consensus sequence, did not...

Topics

Bacterial Proteins
Base Sequence
Binding Sites
DNA
DNA Helicases
DNA Replication
DNA-Binding Proteins
Escherichia coli
Molecular Sequence Data
Mutation
Plasmids

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Identifiers

PMID: 1598213
DOI: 10.1093/nar/20.10.2547
PMCID: PMC312391

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1598213-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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DnaA dependent replication of plasmid R1 occurs in the presence of point mutations that disrupt the dnaA box of oriR.PMID 1598213Read ↗

Mutations in the DnaA binding sites of the replication origin of Escherichia coli.Semantic proximity Read ↗

Differential binding of wild-type and a mutant RepA protein to oriR sequence suggests a model for the initiation of plasmid R1 replication.Semantic proximity Read ↗

A single DnaA box is sufficient for initiation from the P1 plasmid origin.Semantic proximity Read ↗

Site-directed mutations in the repA C-terminal region of plasmid Rts1: pleiotropic effects on the replication and autorepressor functions.Semantic proximity Read ↗

Mutations of DnaA-boxes in the oriR region increase replication frequency of the MiniR1-1 plasmid.Semantic proximity Read ↗

Effects of mutations in the repA gene of plasmid Rts1 on plasmid replication and autorepressor function.Semantic proximity Read ↗

Location of a P1 plasmid replication inhibitor determinant within the initiator gene.Semantic proximity Read ↗

The sequence requirements for a functional Escherichia coli replication origin are different for the chromosome and a minichromosome.Semantic proximity Read ↗

Unique sequence requirements for the P1 plasmid replication origin.Semantic proximity Read ↗

A comprehensive set of DnaA-box mutations in the replication origin, oriC, of Escherichia coli.Semantic proximity Read ↗

Control of replication of plasmid R1: the intergenic region between copA and repA modulates the level of expression of repA.Semantic proximity Read ↗

The DnaA box R4 in the minimal oriC is dispensable for initiation of Escherichia coli chromosome replication.Semantic proximity Read ↗

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