The DnaA box R4 in the minimal oriC is dispensable for initiation of Escherichia coli chromosome replication.

doi:10.1093/nar/23.16.3119

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Article

The DnaA box R4 in the minimal oriC is dispensable for initiation of Escherichia coli chromosome replication.

Nucleic acids research - 25 Aug 1995

Bates D B, Asai T, Cao Y, Chambers M W, Cadwell G W, Boye E, Kogoma T

Abstract excerpt

We have developed a genetic system with which to replace oriC+ on the Escherichia coli chromosome with modified oriC sequences constructed on plasmids. Using this system we have demonstrated that chromosomal oriC can tolerate the insertion of a 2 kb fragment at the HindIII site between DnaA boxes...

Topics

Alleles
Bacterial Proteins
Chromosomes, Bacterial
DNA Replication
DNA, Bacterial
DNA-Binding Proteins
Escherichia coli
Mutagenesis, Insertional
Phenotype
Replication Origin

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