Article
A comprehensive set of DnaA-box mutations in the replication origin, oriC, of Escherichia coli.
Molecular microbiology - 1 Jul 1996
Langer U, Richter S, Roth A, Weigel C, Messer W
Abstract excerpt
We probed the complex between the replication origin, oriC, and the initiator protein DnaA using different types of mutations in the five binding sites for DnaA, DnaA boxes R1-R4 and M: (i) point mutations in individual DnaA boxes and combinations of them; (ii) replacement of the DnaA boxes by a scrambled 9 bp non-box motif; (iii) positional exchange; and (iv) inversion of the DnaA boxes. For each of the five...
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