Article
Unique sequence requirements for the P1 plasmid replication origin.
Research in microbiology - 1 Jan 2000
Brendler T G, Abeles A L, Reaves L D, Austin S J
Abstract excerpt
We have carried out a detailed genetic analysis of the P1 plasmid replication origin and shown that it has four major structural requirements: the DnaA box, a series of five 7-base pair (bp) repeats, a GC-rich spacer and five 19-bp repeats that bind the P1 RepA protein. The origin requires the Dn...
Topics
- Bacterial Proteins
- Base Composition
- Base Sequence
- DNA Helicases
- DNA Replication
- DNA, Bacterial
- DNA-Binding Proteins
- Escherichia coli
- Models, Genetic
- Molecular Sequence Data
- Mutation
- Plasmids