Article
A single DnaA box is sufficient for initiation from the P1 plasmid origin.
Journal of bacteriology - 1 Aug 1990
Abeles A L, Reaves L D, Austin S J
Abstract excerpt
The P1 plasmid replication origin requires the host DnaA protein for function. Two DnaA-binding boxes lie in tandem within the previously defined minimal origin, constituting its left boundary. Three more boxes lie 200 base pairs to the right of these, in the leader region for the P1 repA gene. W...
Topics
- Bacterial Proteins
- Base Sequence
- Chromosome Deletion
- Cloning, Molecular
- DNA Replication
- Escherichia coli
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Plasmids
- Restriction Mapping