Differential binding of wild-type and a mutant RepA protein to oriR sequence suggests a model for the initiation of plasmid R1 replication.

doi:10.1016/0022-2836(92)90864-g

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Article

Differential binding of wild-type and a mutant RepA protein to oriR sequence suggests a model for the initiation of plasmid R1 replication.

Journal of molecular biology - 5 Dec 1992

Giraldo R, Díaz R

Abstract excerpt

DNA replication of the enterobacterial plasmid R1 is initiated by RepA protein. We have developed a new procedure for the purification of RepA from inclusion bodies, which involves CHAPS-mediated solubilization. This method has been also used for the thermosensitive mutant protein RepA2623. The nucleoprotein complexes obtained with both proteins and oriR, the origin of replication, are studied in this paper....

Topics

Bacterial Proteins
Base Sequence
Binding Sites
DNA Helicases
DNA Replication
DNA, Bacterial
DNA-Binding Proteins
Deoxyribonuclease I
Escherichia coli
Free Radicals
Guanidine

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Guanidines

Hydroxides

Macromolecular Substances

Models, Molecular

Molecular Conformation

Molecular Sequence Data

Mutation

Source

Open in PubMed

Identifiers

PMID: 1469713
DOI: 10.1016/0022-2836(92)90864-g

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1469713-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Differential binding of wild-type and a mutant RepA protein to oriR sequence suggests a model for the initiation of plasmid R1 replication.PMID 1469713Read ↗

Structural changes in RepA, a plasmid replication initiator, upon binding to origin DNA.Semantic proximity Read ↗

DnaA dependent replication of plasmid R1 occurs in the presence of point mutations that disrupt the dnaA box of oriR.Semantic proximity Read ↗

Modulation of pPS10 host range by plasmid-encoded RepA initiator protein.Semantic proximity Read ↗

Participation of the Escherichia coli heat shock proteins DnaJ, DnaK, and GrpE in autorepression of the P1 plasmid repA promoter.Semantic proximity Read ↗

Early events in the binding of the pPS10 replication protein RepA to single iteron and operator DNA sequences.Semantic proximity Read ↗

Chaperone-mediated reduction of RepA dimerization is associated with RepA conformational change.Semantic proximity Read ↗

A single DnaA box is sufficient for initiation from the P1 plasmid origin.Semantic proximity Read ↗

Plasmid P1 RepA is homologous to the F plasmid RepE class of initiators.Semantic proximity Read ↗

Analysis of pCU1 replication origins: dependence of oriS on the plasmid-encoded replication initiation protein RepA.Semantic proximity Read ↗

A critical DnaA box directs the cooperative binding of the Escherichia coli DnaA protein to the plasmid RK2 replication origin.Semantic proximity Read ↗

Replication of Enterococcus faecalis pheromone-responding plasmid pAD1: location of the minimal replicon and oriV site and RepA involvement in initiation of replication.Semantic proximity Read ↗

Location of a P1 plasmid replication inhibitor determinant within the initiator gene.Semantic proximity Read ↗

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