Article
Site-directed mutations in the repA C-terminal region of plasmid Rts1: pleiotropic effects on the replication and autorepressor functions.
Journal of bacteriology - 1 May 1990
Zeng H, Hayashi T, Terawaki Y
Abstract excerpt
We induced site-directed mutations near the 3' terminus of the gene repA, which encodes the protein of 288 amino acid residues essential for plasmid Rts1 replication, and obtained seven repA mutants. Three of them contained small deletions at the 3' terminus. Mutant repAz delta C4, which encodes a RepA protein that lacks the C-terminal four amino acids, expressed a high-copy-number phenotype and had lost both...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Coliphages
- DNA Helicases
- DNA Replication
- DNA-Binding Proteins
- Escherichia coli
- Galactokinase
- Genes, Bacterial
- Molecular Sequence Data