Article
Effects of mutations in the repA gene of plasmid Rts1 on plasmid replication and autorepressor function.
Journal of bacteriology - 1 Feb 1990
Terawaki Y, Nozue H, Zeng H, Hayashi T, Kamio Y, Itoh Y
Abstract excerpt
We constructed a system in which wild-type RepA or RepAcop1 protein was supplied in trans in various amounts to coexisting mini-Rts1 plasmids by clones of the repA or repAcop1 gene under the control of the native promoter with or without its operator sequence. RepAcop1 protein which contains a si...
Topics
- Ampicillin Resistance
- Bacterial Proteins
- Cloning, Molecular
- DNA Helicases
- DNA Replication
- DNA-Binding Proteins
- Escherichia coli
- Genes, Bacterial
- Genetic Vectors
- Mutation
- Operon
- Promoter Regions, Genetic
- Proteins
- R Factors
- Restriction Mapping
- Trans-Activators